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In vitro ADME services can effectively provide evaluations of compounds’ absorption, distribution, metabolism, and drug interactions. These services aid in understanding the behavior of compounds within the human body, providing a critical basis for dosage setting, side effect predicting, and risk assessment during the development process.

In Vitro ADME Services

Our in vitro ADME services team offers a very wide services in the field of Physicochemical Properties, Permeability, Metabolic Stability, Drug-Drug Interaction, Transporter, and Protein Binding and Partitioning. We also have a variety of advanced automated liquid workstations and LC-MS/MS analysis technologies to further improve the assays' speed, quality, and throughput. More new modalities with complex structures have emerged from the recent R&D pipelines, including Proteolysis-Targeting Chimeras (PROTACs*), oligonucleotides, and antibody-conjugated drugs (ADCs). There are many challenges in the in vitro ADME study of these new modalities.

Overview

  • Solubility (KS, TS), pKa, logD, logP, and PSA

Physicochemical Property
Drug-drug Interaction
  • Metabolism-mediated drug interactions
  • Transporter-mediated drug interactions


Metabolism
  • Matrix stability (Tissue, plasma, SGF/SIF, buffer, whole blood, GSH reaction, etc.）
  • Metabolic stability (Microsomes, S9, hepatocytes, cytosol, lysosome, mitochondria, recombinase, etc.）

  • PAMPA, Caco-2, MDCK I, MDR1-MDCK I, MDCK II, and MDR1-MDCK II
  • Ex vivo Franz cell-based dermal penetration assay

Permeability
Whole Blood/Plasma Distribution and Protein Binding
  • B/P ratio
  • Dialysis, ultracentrifugation, ultrafiltration, flux dialysis, competition dialysis, etc


Services

Typically, in the Lead Finding (LF) stage, compounds' solubility, lipophilicity, permeability, and microsomal stability are determined first. The obtained data are used to build the structure-property relationship of compounds, screen chemical structure categories, and confirm the priority of multiple structural backbones. At the Lead Optimization (LO) and Pre-Clinical Candidate (PCC) stages, comprehensive in vitro ADME studies are usually required. These include multi-species metabolic stability, plasma protein binding, transporters-related drug interaction, and inhibition of the drug-metabolizing enzymes combined with animal PK data to predict human PK performance. In the Investigational New Drug (IND) stage, a comprehensive evaluation of in vitro ADME properties is required and conducted per the requirements of drug registration authorities. It should be noted that the in vitro ADME screening study is usually not designed for regulatory submission purpose.


What does In Vitro ADME test study?

  • In vitro ADME studies absorption (A), distribution (D), metabolism (M), and excretion (E) properties of the drug candidates in a panel of assays outside of a living organism. The deions of each keyword are as follows. 
    • Absorption: Describes the compound’s ability to pass through physiological barriers such as the intestines, skin, etc.
  • Distribution: Describes how the compound is distributed throughout the body or tissue once it has been taken into the body.
  • Metabolism: Describes how the body breaks down the compound in organs such as liver, kidney, skin or the gut.
  • Excretion: Describes how the body removes the compound.

Our platform can also provide drug-drug interaction evaluation.

What is In Vitro ADME test?
In vitro comes from the Latin term "in glass". It refers to studies that are performed outside of a living organism in a controlled environment, such as a test tube or petri dish. In vitro studies are often contrasted with in vivo ("within the living") studies, which are done in a whole living organism.
The in vitro testing is ideal for testing a potential drug compound with well-controlled testing conditions at an early stage. In vitro testing requires less resources and tends to be much quicker than in vivo models. In vitro testing helps drug developers understand if the drug candidate is suitable to move forward with further investigations.


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